Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7E2] to LST1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LST1 antibody [7E2] - BSA and Azide free
    See all LST1 primary antibodies

  • Description

    Rat monoclonal [7E2] to LST1 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HEK-293 whole cell lysate. IHC-P: Human tonsil tissue. Mouse spleen tissue. Rat thymus tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255833 is the carrier-free version of ab252839. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling LST1 with ab252839 at 4.32µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat thymus tissue is observed. The section was incubated with ab252839 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling LST1 with ab252839 at 4.32µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse spleen tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling LST1 with ab252839 at 4.32µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat thymus tissue is observed. The section was incubated with ab252839 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

  • Western blot - Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)
    Western blot - Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)
    Anti-LST1 antibody [7E2] (ab252839) at 0.432 µg/ml + HEK-293 (human embryonic kidney epithelial cell) lysate at 20 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

    Predicted band size: 11 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 mins.

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 19663701).

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

  • Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)
    Anti-LST1 antibody [7E2] - BSA and Azide free (ab255833)

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