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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Recombinant human CCN3 protein (ab50076)

Recombinant human CCN3 protein (ab50076)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: WB, Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human CCN3 protein
    See all CCN3 proteins and peptides
  • Biological activity

    Determined by a cell proliferation assay using BALB/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 μg/ml

  • Purity

    > 95 % SDS-PAGE.
    ab50076 purity was determined also by HPLC. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M
    • Amino acids

      28 to 357

Preparation and Storage

  • Alternative names

    • CCN family member 3
    • CCN3
    • IBP-9
    • IGF-binding protein 9
    • IGFBP-9
    • IGFBP9
    • Insulin-like growth factor-binding protein 9
    • Nephroblastoma overexpressed
    • Nephroblastoma overexpressed gene protein homolog
    • Nephroblastoma-overexpressed gene protein homolog
    • NOV
    • NOV_HUMAN
    • NovH
    • Protein NOV homolog
    see all
  • Function

    Immediate-early protein likely to play a role in cell growth regulation.
  • Tissue specificity

    Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type.
  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P48745 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Western blot - Recombinant Human CCN3 protein (ab50076)
    Western blot - Recombinant Human CCN3 protein (ab50076)
    Anti-CCN3 antibody (ab10888) at 1 µg/ml + Recombinant human CCN3 protein (ab50076) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Exposure time: 1 minute

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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