Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Cell Biology Other Antibodies

Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821)

Price and availability

314 937 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20885-203] to cleaved C-terminal GSDMD
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Product image
Anti-C2orf69 antibody [EPR13713] - BSA and Azide free (ab240966)
Product image
Anti-CCDC56 antibody (ab122199)
Product image
Anti-DENND1B antibody (ab187903)
Product image
Recombinant Human UNC119B protein (ab123153)

Overview

  • Product name

    Anti-cleaved C-terminal GSDMD antibody [EPR20885-203]
    See all cleaved C-terminal GSDMD primary antibodies
  • Description

    Rabbit monoclonal [EPR20885-203] to cleaved C-terminal GSDMD
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SiHa (treated with 5 µg LPS/10E6 cells by electroporation for 2h.), cell lysate plus concentrated supernatant.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20885-203
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821)
    Western blot - Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821)
    All lanes : Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821) at 1/1000 dilution

    Lane 1 : SiHa (human cervical squamous cell carcinoma), cell lysate plus concentrated cell supernatant
    Lane 2 : SiHa (treated with 5 µg LPS/ 10E6 cells by electroporation for 2h.), cell lysate plus concentrated supernatant.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Predicted band size: 53 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    The image is kindly provided by Dr Feng Shao.

    The molecular weight observed is consistent with literature (PMID: 26375003).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821)
    Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821)

  •  
  • Product image

    Anti-cleaved C-terminal GSDMD antibody [EPR20859-147] (ab255603)

    Applications: WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)

  •  
  • Product image

    Mouse Hemoglobin ELISA Kit (Hba) (ab254517)

  •  
  • Product image

    Anti-Wnt3a antibody - C-terminal (ab198220)

  •  
  • Product image

    Anti-Kappa light chain antibody [HP6053-L1C1] - BSA and Azide free (ab233889)

  •  
  • Product image

    Anti-CCL27 antibody - BSA and Azide free (Capture) (ab242461)

  •  
  • Product image

    Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.