Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19839-8] to Prosurfactant Protein C
  • Suitable for: IHC-P, WB, IHC-Fr, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Prosurfactant Protein C antibody [EPR19839-8]
    See all Prosurfactant Protein C primary antibodies

  • Description

    Rabbit monoclonal [EPR19839-8] to Prosurfactant Protein C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung tissue lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)
    Western blot - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)
    Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521) at 1/1000 dilution + Mouse lung tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking and diluting buffer and concentration: This target has a precursor which contains an exceedingly hybrophobic region of 34 amino acids that comprises most of the mature Spftc, and a unique poly-valine domain. The staining pattern of our antibody is similar with published paper (PMID: 20378731 and PMID: 22461427). 15 seconds  Exposure time: 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Prosurfactant Protein C with ab270521 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Type II alveolar cells in mouse lung. The section was incubated with ab270521 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)
    Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling Prosurfactant Protein C with ab270521 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)
    Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)

    Prosurfactant Protein C was immunoprecipitated from 0.35 mg Mouse lung tissue lysate 1with ab270521 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab270521 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse lung tissue lysate 10 ug.

    Lane 2: ab270521 IP in Mouse lung tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270521 in mouse lung tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Prosurfactant Protein C with ab270521 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The Pulmonary surfactant-associated protein C is a peptide secreted by the alveolar type II pneumocytes of the lung (PMID: 16769199). Negative control: No staining on mouse spleen. The section was incubated with ab270521 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)
    Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521)

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