Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)
![Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)](https://www.abcam.com/ps/products/251/ab251491/Images/ab251491-408313-anti-lipoprotein-a-antibody-epr19731-bsa-and-azide-free-western-blot.jpg "Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19731] to Lipoprotein a - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free
See all Lipoprotein a primary antibodies -
Description
Rabbit monoclonal [EPR19731] to Lipoprotein a - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251491 is the carrier-free version of ab208184. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251491 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19731 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)All lanes : Anti-Lipoprotein a antibody [EPR19731] (ab208184) at 1/5000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human liver tissue lysate
Lane 4 : Human blood
Lane 5 : Human adipose tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 501 kDa
Observed band size: 100-500 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab208184, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 9590382, 10484779 and 10703674).
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![Western blot - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)](https://www.abcam.com/ps/products/251/ab251491/Images/ab251491-406172-anti-lipoprotein-a-antibody-epr19731-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)All lanes : Anti-Lipoprotein a antibody [EPR19731] (ab208184) at 1/5000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human liver lysate
Lane 4 : Human blood
Lane 5 : Human adipose lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 501 kDa
Observed band size: 100-500 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab208184, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 9590382, 10484779 and 10703674).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)](https://www.abcam.com/ps/products/251/ab251491/Images/ab251491-2-anti-lipoprotein-a-antibody-epr19731-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)This data was developed using ab208184, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)](https://www.abcam.com/ps/products/251/ab251491/Images/ab251491-1-anti-lipoprotein-a-antibody-epr19731-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)This data was developed using ab208184, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on blood vessels of human glioma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)](https://www.abcam.com/ps/products/251/ab251491/Images/ab251491-5-benefits-of-recombinant-antibodies.png)
Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (ab251491)