Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UNQ511 antibody [EPR23557-128] (ab270397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23557-128] to UNQ511
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-UNQ511 antibody [EPR23557-128]
    See all UNQ511 primary antibodies

  • Description

    Rabbit monoclonal [EPR23557-128] to UNQ511

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human colon and colon cancer tissue lysates. Human Human large intestine tissue lysates treated with PNGase F IHC: Human colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23557-128

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UNQ511 antibody [EPR23557-128] (ab270397)
    Western blot - Anti-UNQ511 antibody [EPR23557-128] (ab270397)
    All lanes : Anti-UNQ511 antibody [EPR23557-128] (ab270397) at 1/1000 dilution

    Lane 1 : Human colon tissue lysate
    Lane 2 : Human colon cancer tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Exposure time: 3 minutes

    The molecular weight observed is consistent with what has been described in the literature (PMID:27027293)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNQ511 antibody [EPR23557-128] (ab270397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNQ511 antibody [EPR23557-128] (ab270397)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UNQ511 with ab270397 at 1/250 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in apical surface of epithelial cells of human colon (PMID: 27027293) is observed.  Counterstained with Hematoxylin

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Western blot - Anti-UNQ511 antibody [EPR23557-128] (ab270397)
    Western blot - Anti-UNQ511 antibody [EPR23557-128] (ab270397)
    All lanes : Anti-UNQ511 antibody [EPR23557-128] (ab270397) at 1/1000 dilution

    Lane 1 : Human large intestine tissue lysate
    Lane 2 : Human large intestine tissue lysate treated with PNGase F

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 100,35 kDa why is the actual band size different from the predicted?



    Exposure time: 3 minutes

    The molecular weight observed is consistent with what has been described in the literature (PMID:27027293).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNQ511 antibody [EPR23557-128] (ab270397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNQ511 antibody [EPR23557-128] (ab270397)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UNQ511 with ab270397 at 1/250 dilution (2 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Negative control: no staining in human liver (PMID: 27027293).

    Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Anti-UNQ511 antibody [EPR23557-128] (ab270397)
    Anti-UNQ511 antibody [EPR23557-128] (ab270397)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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