Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

Order now and get it on Tuesday March 16, 2021

Anti-UNQ511 antibody [EPR23557-128] (ab270397)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23557-128] to UNQ511
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-UNQ511 antibody [EPR23557-128]
See all UNQ511 primary antibodies
Description

Rabbit monoclonal [EPR23557-128] to UNQ511
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human colon and colon cancer tissue lysates. Human Human large intestine tissue lysates treated with PNGase F IHC: Human colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23557-128
Isotype

IgG
Research areas

Western blot - Anti-UNQ511 antibody [EPR23557-128] (ab270397)

All lanes : Anti-UNQ511 antibody [EPR23557-128] (ab270397) at 1/1000 dilution

Lane 1 : Human colon tissue lysate
Lane 2 : Human colon cancer tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 100 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

The molecular weight observed is consistent with what has been described in the literature (PMID:27027293)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNQ511 antibody [EPR23557-128] (ab270397)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UNQ511 with ab270397 at 1/250 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in apical surface of epithelial cells of human colon (PMID: 27027293) is observed. Counterstained with Hematoxylin

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Western blot - Anti-UNQ511 antibody [EPR23557-128] (ab270397)

All lanes : Anti-UNQ511 antibody [EPR23557-128] (ab270397) at 1/1000 dilution

Lane 1 : Human large intestine tissue lysate
Lane 2 : Human large intestine tissue lysate treated with PNGase F

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 100,35 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

The molecular weight observed is consistent with what has been described in the literature (PMID:27027293).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNQ511 antibody [EPR23557-128] (ab270397)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UNQ511 with ab270397 at 1/250 dilution (2 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Negative control: no staining in human liver (PMID: 27027293).

Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Anti-UNQ511 antibody [EPR23557-128] (ab270397)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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