Anti-LIMPII antibody [EPR12080] (ab176317)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12080] to LIMPII
- Suitable for: WB, Flow Cyt, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LIMPII antibody [EPR12080]
See all LIMPII primary antibodies -
Description
Rabbit monoclonal [EPR12080] to LIMPII -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human LIMPII aa 50-150. The exact sequence is proprietary.
Database link: Q14108 -
Positive control
- WB: HeLa and Y79 cell lysate. Rat eyeball and muscle lysate. Mouse liver lysate. IP: Y79 cell lysate. Flow Cyt: Permeabilized HeLa cells. IHC-P:Human kidney and liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12080 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-LIMPII antibody [EPR12080] (ab176317) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2 : Y79 (Human retinoblastoma cell line) lysate
Lane 3 : Rat eyeball lysate
Lane 4 : Rat muscle lysate
Lane 5 : Mouse liver lysate
Predicted band size: 54 kDa
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Western blot analysis on immunoprecipitation pellet from Y79 (Human retinoblastoma cell line) cell lysate labeling LIMPII using ab176317 at a 1/10 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labling LIMPII using ab176317 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labling LIMPII using ab176317 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized HeLa cells labeling LIMPII using ab176317 at a 1/10 dilution (red) or a rabbit IgG control (green).
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