Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-LIMPII antibody [EPR12080] (ab176317)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12080] to LIMPII
Suitable for: WB, Flow Cyt, IP, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-LIMPII antibody [EPR12080]
See all LIMPII primary antibodies
Description

Rabbit monoclonal [EPR12080] to LIMPII
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human LIMPII aa 50-150. The exact sequence is proprietary.
Database link: Q14108
Positive control
- WB: HeLa and Y79 cell lysate. Rat eyeball and muscle lysate. Mouse liver lysate. IP: Y79 cell lysate. Flow Cyt: Permeabilized HeLa cells. IHC-P:Human kidney and liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12080
Isotype

IgG
Research areas

Western blot - Anti-LIMPII antibody [EPR12080] (ab176317)

All lanes : Anti-LIMPII antibody [EPR12080] (ab176317) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2 : Y79 (Human retinoblastoma cell line) lysate
Lane 3 : Rat eyeball lysate
Lane 4 : Rat muscle lysate
Lane 5 : Mouse liver lysate

Predicted band size: 54 kDa
Immunoprecipitation - Anti-LIMPII antibody [EPR12080] (ab176317)

Western blot analysis on immunoprecipitation pellet from Y79 (Human retinoblastoma cell line) cell lysate labeling LIMPII using ab176317 at a 1/10 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labling LIMPII using ab176317 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)

Immunohistochemical analysis of paraffin-embedded human liver tissue labling LIMPII using ab176317 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-LIMPII antibody [EPR12080] (ab176317)

Flow cytometric analysis of permeabilized HeLa cells labeling LIMPII using ab176317 at a 1/10 dilution (red) or a rabbit IgG control (green).
Anti-LIMPII antibody [EPR12080] (ab176317)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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