Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12080] to LIMPII - BSA and Azide free
  • Suitable for: Flow Cyt, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LIMPII antibody [EPR12080] - BSA and Azide free
    See all LIMPII primary antibodies

  • Description

    Rabbit monoclonal [EPR12080] to LIMPII - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, IHC-P, WBmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and Y79 cell lysate. Rat eyeball and muscle lysate. Mouse liver lysate. IP: Y79 cell lysate. Flow Cyt: Permeabilized HeLa cells. IHC-P:Human kidney and liver tissue.

  • General notes

    Ab240186 is the carrier-free version of ab176317. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240186 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)
    Immunoprecipitation - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    Western blot analysis on immunoprecipitation pellet from Y79 (Human retinoblastoma cell line) cell lysate labeling LIMPII using ab176317 at a 1/10 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176317).

  • Flow Cytometry - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)
    Flow Cytometry - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    Flow cytometric analysis of permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling LIMPII using ab176317 at a 1/10 dilution (red) or a rabbit IgG control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176317).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labling LIMPII using ab176317 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176317).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labling LIMPII using ab176317 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176317).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)
    Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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