Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-L-Kynurenine Hydrolase antibody [062] - BSA and Azide free (ab276053)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [062] to L-Kynurenine Hydrolase - BSA and Azide free
  • Suitable for: WB, ICC, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-L-Kynurenine Hydrolase antibody [062] - BSA and Azide free
    See all L-Kynurenine Hydrolase primary antibodies

  • Description

    Rabbit monoclonal [062] to L-Kynurenine Hydrolase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human L-Kynurenine Hydrolase aa 1-465.
    Database link: Q16719

  • Positive control

    • ICC: A549 cells. IP: A549 cells. WB: A549 cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-L-Kynurenine Hydrolase antibody [062] (ab276053)
    Immunocytochemistry - Anti-L-Kynurenine Hydrolase antibody [062] (ab276053)

    Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilized A549 (Human lung carcinoma cell line) cells labeling L-Kynurenine Hydrolase with ab276053 at 1/60 dilution, 4°C overnight, folloed by Goat Anti-rabbit IgG Alexa Fluor 488-conjugated secondary antibody (green). Positive staining was localized to Cytoplasm. Counterstained with DAPI (blue). Cells were blocked with 10% serum prior incubation.

  • Immunoprecipitation - Anti-L-Kynurenine Hydrolase antibody [062] (ab276053)
    Immunoprecipitation - Anti-L-Kynurenine Hydrolase antibody [062] (ab276053)

    KYNU-HIS was immunoprecipitated using 0.5 mg of A549 (Human lung carcinoma cell line) whole cell lysate with 0.5 µL ab276053 and 15 µl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276053 at 1/500 dilution. Clean-Blot IP Detection Reagent (HRP), was used as secondary antibody at 1/1000 dilution.

    Developed using the DAB staining technique.
    Performed under reducing conditions.

  • Western blot - Anti-L-Kynurenine Hydrolase antibody [062] (ab276053)
    Western blot - Anti-L-Kynurenine Hydrolase antibody [062] (ab276053)
    Anti-L-Kynurenine Hydrolase antibody [062] - BSA and Azide free (ab276053) at 1/500 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 52 kDa



    Developed using the Odyssey technique.
    Performed under reducing conditions.

  • Anti-L-Kynurenine Hydrolase antibody [062] - BSA and Azide free (ab276053)
    Anti-L-Kynurenine Hydrolase antibody [062] - BSA and Azide free (ab276053)

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