Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22990-12] to NKR-P1C - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free
    See all NKR-P1C primary antibodies

  • Description

    Rabbit monoclonal [EPR22990-12] to NKR-P1C - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Mouse splenocytes. Flow cyt: Mouse splenocytes.

  • General notes

    ab263867 is the carrier-free version of ab242134. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab263867 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)
    Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)

    Flow cytometric analysis of mouse splenocyte cells labeling NKR-P1C with ab242134 at 1/500 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab242134 (Right), then stained with anti-CD49b conjugated to PE. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242134).

  • Immunocytochemistry/ Immunofluorescence - Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)
    Immunocytochemistry/ Immunofluorescence - Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocyte cells labeling NK1.1 with ab242134 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242134).

  • Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)
    Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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