Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)
![Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)](https://www.abcam.com/ps/products/263/ab263867/Images/ab263867-355793-242134FC1anti-nk11-antibody-epr22990-12-flowcytometry-spleen-mouse.jpg "Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22990-12] to NKR-P1C - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free
See all NKR-P1C primary antibodies -
Description
Rabbit monoclonal [EPR22990-12] to NKR-P1C - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Mouse splenocytes. Flow cyt: Mouse splenocytes.
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General notes
ab263867 is the carrier-free version of ab242134. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab263867 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22990-12 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)
Flow cytometric analysis of mouse splenocyte cells labeling NKR-P1C with ab242134 at 1/500 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab242134 (Right), then stained with anti-CD49b conjugated to PE. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242134).
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![Immunocytochemistry/ Immunofluorescence - Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)](https://www.abcam.com/ps/products/263/ab263867/Images/ab263867-1-anti-nk1-1-antibody-epr22990-12-immunocytochemistry-spleen-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocyte cells labeling NK1.1 with ab242134 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242134).
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![Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)](https://www.abcam.com/ps/products/263/ab263867/Images/ab263867-3-benefits-of-recombinant-antibodies.png)
Anti-NKR-P1C antibody [EPR22990-12] - BSA and Azide free (ab263867)
