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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules

Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)

Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11843] to TUBB2A+TUBB2B - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt (Intra), IP, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free
    See all TUBB2A+TUBB2B primary antibodies
  • Description

    Rabbit monoclonal [EPR11843] to TUBB2A+TUBB2B - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), IP, WBmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human TUBB2A+TUBB2B. The exact sequence is proprietary.
    Database link: Q13885

  • General notes

    ab249602 is the carrier-free version of ab170931.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11843
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin

Images

  • Western blot - Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    Western blot - Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    All lanes : Anti-TUBB2A + TUBB2B antibody [EPR11843] (ab170931) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 50 kDa



    This data was developed using ab170931, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    This data was developed using ab170931, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TUBB2A with ab170931 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    Immunoprecipitation - Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    This data was developed using ab170931, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from 293T cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170931 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.Lane 1 Primary: ab170931, 1/10000 dilution
    Sample: Immunoprecipitation pellet from 293T cell lysate
    Secondary: HRP-conjugated anti-rabbit IgG Lane 2 Primary: ab170931, 1/10000 dilution
    Sample: 1X PBS (negative control)
    Secondary: HRP-conjugated anti-rabbit IgG
  • Flow Cytometry - Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    Flow Cytometry - Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    This data was developed using ab170931, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells labeling TUBB2A with ab170931 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
  • Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)
    Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free (ab249602)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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