Anti-KIF5A antibody (ab5628)
Key features and details
- Rabbit polyclonal to KIF5A
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KIF5A antibody
See all KIF5A primary antibodies -
Description
Rabbit polyclonal to KIF5A -
Host species
Rabbit -
Specificity
This antibody is specific for KIF5A and does not detect other kinesin isotypes.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse KIF5A aa 1007-1027.
Sequence:CGYEAEDQAKLFPLHQETAAS
(Peptide available asab41781) -
General notes
This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KIF5A antibody (ab5628)Anti-KIF5A antibody (ab5628) + mouse neuronal cell extract
Secondary
HRP-conjugated anti rabbit antibody
Predicted band size: 117 kDaShows a Western blot of KIF5A on mouse neuronal cell extract using ab5628.
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Western blot - Anti-KIF5A antibody (ab5628)Anti-KIF5A antibody (ab5628) + human retinal extract
Secondary
HRP-conjugated anti-rabbit antibody
Predicted band size: 117 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)ICC/IF image of ab5628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5628, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
