Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KRIT1 antibody [EPR16560] (ab196025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16560] to KRIT1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-KRIT1 antibody [EPR16560]
    See all KRIT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16560] to KRIT1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain and Mouse brain lysates; SH-SY5Y, Raji, NIH/3T3 and HUVEC cell lysates; HUVEC cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)
    Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)
    All lanes : Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) cell lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 84 kDa
    Observed band size: 84 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)
    Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)
    All lanes : Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 84 kDa
    Observed band size: 84 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)
    Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)
    Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilution + HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 84 kDa
    Observed band size: 84 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-KRIT1 antibody [EPR16560] (ab196025)
    Immunocytochemistry/ Immunofluorescence - Anti-KRIT1 antibody [EPR16560] (ab196025)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (Human umbilical vein endothelial cell line) cells labeling KRIT1 with ab196025 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HUVEC cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab196025 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-KRIT1 antibody [EPR16560] (ab196025)
    Anti-KRIT1 antibody [EPR16560] (ab196025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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