Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 03, 2021

Anti-KRIT1 antibody [EPR16560] (ab196025)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16560] to KRIT1
Suitable for: WB, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-KRIT1 antibody [EPR16560]
See all KRIT1 primary antibodies
Description

Rabbit monoclonal [EPR16560] to KRIT1
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain and Mouse brain lysates; SH-SY5Y, Raji, NIH/3T3 and HUVEC cell lysates; HUVEC cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16560
Isotype

IgG
Research areas

Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)

All lanes : Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa
Observed band size: 84 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)

All lanes : Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa
Observed band size: 84 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025)

Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilution + HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa
Observed band size: 84 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-KRIT1 antibody [EPR16560] (ab196025)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (Human umbilical vein endothelial cell line) cells labeling KRIT1 with ab196025 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HUVEC cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab196025 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Anti-KRIT1 antibody [EPR16560] (ab196025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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