Anti-GTPBP4/NOG1 antibody (ab184124)
Key features and details
- Rabbit polyclonal to GTPBP4/NOG1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GTPBP4/NOG1 antibody
See all GTPBP4/NOG1 primary antibodies -
Description
Rabbit polyclonal to GTPBP4/NOG1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human GTPBP4/NOG1 aa 272-557. The exact sequence is proprietary.
Database link: Q9BZE4 -
Positive control
- 293T , A431 , HeLa , HepG2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1, C2C12 , PC-12 , Rat2 whole cell lysates, Hela cells, mouse ovary tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GTPBP4/NOG1 antibody (ab184124)All lanes : Anti-GTPBP4/NOG1 antibody (ab184124) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Predicted band size: 74 kDa7.5% SDS PAGE
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Western blot - Anti-GTPBP4/NOG1 antibody (ab184124)All lanes : Anti-GTPBP4/NOG1 antibody (ab184124) at 1/500 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH-3T3 whole cell lysate
Lane 5 : BCL-1 whole cell lysate
Lane 6 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa7.5% SDS PAGE
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Western blot - Anti-GTPBP4/NOG1 antibody (ab184124)All lanes : Anti-GTPBP4/NOG1 antibody (ab184124) at 1/500 dilution
Lane 1 : PC-12 whole cell lysate
Lane 2 : Rat2 whole cell lysate/ whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-GTPBP4/NOG1 antibody (ab184124)Immunocytochemical analysis of Hela cells fixed in 4% paraformaldehyde at room temperature for 15 mins labeling GTPBP4/NOG1 with ab184124 at 1/500 dilution (green) and counterstained with Hoechst 33342 (blue)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTPBP4/NOG1 antibody (ab184124)Immunohistochemical staining of paraffin-embedded mouse ovary tissue showing nuclear staining labeling GTPBP4/NOG1 with ab184124 at 1/500 dilution
