Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RAB45 antibody [EPR16349] (ab194827)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16349] to RAB45
Suitable for: ICC/IF, Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-RAB45 antibody [EPR16349]
See all RAB45 primary antibodies
Description

Rabbit monoclonal [EPR16349] to RAB45
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- A549, A431, K562, C6, RAW 264.7, NIH 3T3 lysates; Human testis, mouse brain, heart and spleen, rat brain and heart lysates; HeLa and K562 cells.
General notes
This product was previously labelled as RASEF

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16349
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (ab194827)

Immunofluorescent analysis of HeLa cells labeling RAB45 with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HeLa cell line was observed.
Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)

All lanes : Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : C6 cell lysate
Lane 7 : RAW 264.7 cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa
Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)

Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution + K562 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa
Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)

All lanes : Anti-RAB45 antibody [EPR16349] (ab194827) at 1/1000 dilution

Lane 1 : A549 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa
Flow Cytometry - Anti-RAB45 antibody [EPR16349] (ab194827)

Flow cytometry analysis of K562 cells labeling RAB45 using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody: Blue. Rabbit monoclonal IgG was used as isotype control (Black).
Anti-RAB45 antibody [EPR16349] (ab194827)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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