Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB45 antibody [EPR16349] (ab194827)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16349] to RAB45
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RAB45 antibody [EPR16349]
    See all RAB45 primary antibodies

  • Description

    Rabbit monoclonal [EPR16349] to RAB45

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A549, A431, K562, C6, RAW 264.7, NIH 3T3 lysates; Human testis, mouse brain, heart and spleen, rat brain and heart lysates; HeLa and K562 cells.

  • General notes

     

     

     This product was previously labelled as RASEF

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (ab194827)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] (ab194827)

    Immunofluorescent analysis of HeLa cells labeling RAB45 with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HeLa cell line was observed.

  • Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)
    Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)
    All lanes : Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : C6 cell lysate
    Lane 7 : RAW 264.7 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa

  • Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)
    Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)
    Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution + K562 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa

  • Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)
    Western blot - Anti-RAB45 antibody [EPR16349] (ab194827)
    All lanes : Anti-RAB45 antibody [EPR16349] (ab194827) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa

  • Flow Cytometry - Anti-RAB45 antibody [EPR16349] (ab194827)
    Flow Cytometry - Anti-RAB45 antibody [EPR16349] (ab194827)

    Flow cytometry analysis of K562 cells labeling RAB45 using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody: Blue. Rabbit monoclonal IgG was used as isotype control (Black).

  • Anti-RAB45 antibody [EPR16349] (ab194827)
    Anti-RAB45 antibody [EPR16349] (ab194827)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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