A titer ELISA using ab224181. The plate was coated with different amounts of human IgG3. A serial dilution of ab224181 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA of human immunoglobulins shows ab224181 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab224181 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

Sandwich ELISA using ab224181 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

Sandwich ELISA using ab224181 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by a HRP conjugated streptavidin.

Human lymphoid tissue stained for human IgG3 using ab224181 at 5 µg/ml in immunohistochemical analysis.