Anti-KCNC2 antibody [S410-17] (ab241999)
![Anti-KCNC2 antibody [S410-17] (ab241999)](https://www.abcam.com/ps/products/241/ab241999/Images/ab241999-331823-99b.jpg "Anti-KCNC2 antibody [S410-17] (ab241999)")
Key features and details
- Mouse monoclonal [S410-17] to KCNC2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-KCNC2 antibody [S410-17]
See all KCNC2 primary antibodies -
Description
Mouse monoclonal [S410-17] to KCNC2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseRat
-
Immunogen
Fusion protein corresponding to Rat KCNC2 aa 474-613. (Cytoplasmic C-terminus)
Database link: P22462 -
Positive control
- ICC/IF: SK-N-BE cells; WB: Rat brain tissue lysate; rat brain membrane lysate; mouse brain lysate; IHC-P: Human brain cortex tissue.
-
General notes
This product was previously labelled as Kv3.2
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S410-17 -
Isotype
IgG1 -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-KCNC2 antibody [S410-17] (ab241999)
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for KCNC2 (green, C) using ab241999 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution (1 hr at RT). Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNC2 antibody [S410-17] (ab241999)](https://www.abcam.com/ps/products/241/ab241999/Images/ab241999-451273-anti-kcnc2-antibody-s410-17-immunohistochemistry-brain-cortex-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNC2 antibody [S410-17] (ab241999)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain cortex tissue labeling KCNC2 with ab241999.
-
![Western blot - Anti-KCNC2 antibody [S410-17] (ab241999)](https://www.abcam.com/ps/products/241/ab241999/Images/ab241999-331822-99a.jpg)
Western blot - Anti-KCNC2 antibody [S410-17] (ab241999)All lanes : Anti-KCNC2 antibody [S410-17] (ab241999) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain membrane lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/200 dilution
Predicted band size: 70 kDaPrimary incubation for 16 hours at 4°C.
Secondary incubation for 1 hr at RT.
Blocked for 1 hr at RT.
