Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR140(2)] to JNK1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free
    See all JNK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR140(2)] to JNK1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247935 is the carrier-free version of ab110724. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247935 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)
    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

    This data was developed using ab110724, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: MCF7 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab110724 (unpurified) was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)
    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)
    All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/2000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab110724, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)
    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

    This data was developed using ab110724, the same antibody clone in a different buffer formulation.

    Lane 1 Wild-type HAP1 cell lysate (20 µg)

    Lane 2 JNK1 knockout HAP1 cell lysate (20 µg)

    Lane 3 HeLa cell lysate (20 µg)

    Lane 4 MCF7 cell lysate (20 µg)

    Lanes 1 - 4 Merged signal (red and green).

    Green - target observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

    This western blot image is a comparison between ab110724 and a competitor's top cited mouse monoclonal antibody.

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)
    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)
    All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 48 kDa



    This data was developed using ab110724, the same antibody clone in a different buffer formulation.

     

  • Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)
    Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

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