Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5221] to IQGAP1 - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free
    See all IQGAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5221] to IQGAP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247821 is the carrier-free version of ab109292 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247821 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    Western blot - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)

    This data was developed using ab109292, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: IQGAP1 knockout HAP1 cell lysate (20 µg)

    Lane 3: HEK293 cell lysate (20 µg)

    Lane 4: HeLa cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109292 observed at 190 kDa. Red - loading control, ab7291, observed at 52 kDa.

    ab109292 was shown to specifically react with IQGAP1 when IQGAP1 knockout samples were used. Wild-type and IQGAP1 knockout samples were subjected to SDS-PAGE. ab109292 and ab7291 (loading control to alpha tubulin) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    Flow Cytometry - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    This data was developed using ab109292, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IQGAP1 with unpurified ab109292 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Immunocytochemistry - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    Immunocytochemistry - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    This data was developed using ab109292, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling IQGAP1 with Purified ab109292 at 1/500 dilution (5 µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
  • Western blot - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    Western blot - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    All lanes : Anti-IQGAP1 antibody [EPR5221] (ab109292) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Cos-1 cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 189 kDa



    This data was developed using ab109292, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    This data was developed using ab109292, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human uterus tissue using ab109292 at a dilution of 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)
    Anti-IQGAP1 antibody [EPR5221] - BSA and Azide free (ab247821)

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