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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337)

Price and availability

237 878 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP974(2)Y] to Integrin beta 1 (phospho Y783)
  • Suitable for: WB, Dot blot
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y]
    See all Integrin beta 1 primary antibodies
  • Description

    Rabbit monoclonal [EP974(2)Y] to Integrin beta 1 (phospho Y783)
  • Host species

    Rabbit
  • Specificity

    ab62337 recognises Integrin beta 1 phosphorylated at Tyrosine 783.
  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Integrin beta 1 (phospho Y783).

  • Positive control

    • WB: U937 cell lysates treated with pervanadate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP974(2)Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

Images

  • Western blot - Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337)
    Western blot - Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337)
    All lanes : Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337) at 1/1000 dilution

    Lane 1 : U937 whole cell lysate
    Lane 2 : U937 treated with 10mM pervanadate for 15min whole cell lysate
    Lane 3 : U937 treated with 10mM pervanadate for 15min whole cell lysate, the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 88 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337)
    Dot Blot - Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337)

    Dot blot analysis of Integrin beta 1 (phospho Y783) peptide (Lane 1) and Integrin beta 1 non-phospho peptide (Lane 2) labelling Integrin beta 1 (phospho Y783) with ab62337 at a dilution of 1/1000 dilution. A goat anti-rabbit IgG, (H+L), peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000 dilution.

    Blocking buffer: 5% NFDM/TBST.
    Dilution buffer: 5% NFDM /TBST .

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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