Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337)
Key features and details
- Rabbit monoclonal [EP974(2)Y] to Integrin beta 1 (phospho Y783)
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y]
See all Integrin beta 1 primary antibodies -
Description
Rabbit monoclonal [EP974(2)Y] to Integrin beta 1 (phospho Y783) -
Host species
Rabbit -
Specificity
ab62337 recognises Integrin beta 1 phosphorylated at Tyrosine 783. -
Tested applications
Suitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Integrin beta 1 (phospho Y783).
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Positive control
- WB: U937 cell lysates treated with pervanadate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP974(2)Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (ab62337) at 1/1000 dilution
Lane 1 : U937 whole cell lysate
Lane 2 : U937 treated with 10mM pervanadate for 15min whole cell lysate
Lane 3 : U937 treated with 10mM pervanadate for 15min whole cell lysate, the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of Integrin beta 1 (phospho Y783) peptide (Lane 1) and Integrin beta 1 non-phospho peptide (Lane 2) labelling Integrin beta 1 (phospho Y783) with ab62337 at a dilution of 1/1000 dilution. A goat anti-rabbit IgG, (H+L), peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000 dilution.
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% NFDM /TBST .