Antibodies, Proteins, Kits and Reagents for Life Science

HRP Anti-Integrin beta 1 antibody [EPR1040Y] (ab216295)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR1040Y] to Integrin beta 1
Suitable for: WB
Knockout validated
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-Integrin beta 1 antibody [EPR1040Y]
See all Integrin beta 1 primary antibodies
Description

HRP Rabbit monoclonal [EPR1040Y] to Integrin beta 1
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U937, HeLa, U87-MG, A431 and HAP1 WT (shows pos., Integrin beta 1 knockout HAP1 KO shows neg).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1040Y
Isotype

IgG
Research areas

Western blot - HRP Anti-Integrin beta 1 antibody [EPR1040Y] (ab216295)

All lanes : HRP Anti-Integrin beta 1 antibody [EPR1040Y] (ab216295) at 1/5000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ITGB1 (Integrin beta 1) knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 88 kDa

Exposure time: 20 minutes

ab216295 was shown to specifically react with Integrin beta 1 in wild-type HAP1 cells as signal was lost in ITGB1 (Integrin beta 1) knockout cells. Wild-type and ITGB1 (Integrin beta 1) knockout samples were subjected to SDS-PAGE. Ab216295 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
Western blot - HRP Anti-Integrin beta 1 antibody [EPR1040Y] (ab216295)

All lanes : HRP Anti-Integrin beta 1 antibody [EPR1040Y] (ab216295) at 1/5000 dilution

Lane 1 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate at 10 µg
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5 : HAP1 WT at 20 µg
Lane 6 : Integrin beta 1 knockout HAP1 at 20 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 88 kDa

Exposure time: 20 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab216295 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
HRP Anti-Integrin beta 1 antibody [EPR1040Y] (ab216295)

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