Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD24 antibody [EPR19925] - BSA and Azide free (ab228455)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19925] to CD24 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD24 antibody [EPR19925] - BSA and Azide free
    See all CD24 primary antibodies

  • Description

    Rabbit monoclonal [EPR19925] to CD24 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells.

  • General notes

    Ab228455 is the carrier-free version of ab202073. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab228455 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Flow Cytometry - Anti-CD24 antibody [EPR19925] - BSA and Azide free (ab228455)
    Flow Cytometry - Anti-CD24 antibody [EPR19925] - BSA and Azide free (ab228455)

    Flow cytometric analysis of MDA-MB-231 (human breast adenocarcinoma cell line, Left) and MCF7 breast adenocarcinoma cell line, Right) cell lines labeling CD24 with ab202073 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on total viable cells. Negative control: MDA-MB-231 (PMID: 18433506 and 22934288).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202073).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR19925] - BSA and Azide free (ab228455)
    Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR19925] - BSA and Azide free (ab228455)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) and MDA-MB-231 (human breast adenocarcinoma cell line) cells labeling CD24 with ab202073 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on MCF7 cell line.

    Negative control: MDA-MB-231 (PMID: 18433506).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202073).

  • Anti-CD24 antibody [EPR19925] - BSA and Azide free (ab228455)
    Anti-CD24 antibody [EPR19925] - BSA and Azide free (ab228455)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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