Anti-Vimentin antibody (ab137321)
Key features and details
- Rabbit polyclonal to Vimentin
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Vimentin antibody
See all Vimentin primary antibodies -
Description
Rabbit polyclonal to Vimentin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseRatHumanIP HumanWB MouseHuman
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Immunogen
Recombinant fragment within Human Vimentin (internal sequence). The exact sequence is proprietary.
Database link: P08670 -
Positive control
- WB: HEK-293T, HeLa and NIH/3T3 whole cell lysates. U87-MG, SK-N-SH. IMR32 and SK-N-AS whole cell extracts. ICC/IF: HeLa cells. IHC-P: Adult mouse retina tissue. U373 xenograft tissue and RT2 rat xenograft tissue. IP: HeLa whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Vimentin antibody (ab137321)All lanes : Anti-Vimentin antibody (ab137321) at 1/50000 dilution
Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extracts
Lane 3 : IMR32 whole cell extracts
Lane 4 : SK-N-AS whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa10% SDS-PAGE gel
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded adult mouse retina tissue stained for Vimentin with ab137321 (1/250 dilution) (green) in immunohistochemical analysis.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded rat testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded mouse testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded mouse ovary tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab137321 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
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Immunoprecipitation - Anti-Vimentin antibody (ab137321)Vimentin was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 5 µg of ab137321.
Lane 1: HeLa whole cell extracts (Input).
Lane 2: Control IgG in HeLa whole cell extracts.
Lane 3: Vimentin IP in HeLa whole cell extracts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Paraffin-embedded human lung carcinoma tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Immunohistochemical analysis of paraffin-embedded, formalin-fixed RT2 rat xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
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Western blot - Anti-Vimentin antibody (ab137321)Anti-Vimentin antibody (ab137321) at 1/3000 dilution + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 54 kDa7.5% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)Confocal immunofluorescence analysis of methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin, using ab137321 (green) at a 1/500 dilution. Alpha-tubulin filaments were labelled with an anti-alpha tubilin antibody (red) at a 1/2000 dilution.
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Western blot - Anti-Vimentin antibody (ab137321)All lanes : Anti-Vimentin antibody (ab137321) at 1/3000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Predicted band size: 54 kDa10% SDS PAGE
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Western blot - Anti-Vimentin antibody (ab137321)Anti-Vimentin antibody (ab137321) at 1/1000 dilution + NIH-3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS Page -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)Immunohistochemical analysis of paraffin-embedded, formalin-fixed U373 xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
