Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20449] to Lhx2/LH2
Suitable for: WB, IHC-Fr, IP, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Lhx2/LH2 antibody [EPR20449]
See all Lhx2/LH2 primary antibodies
Description

Rabbit monoclonal [EPR20449] to Lhx2/LH2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
IP	Rat
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426); E18 rat brain lysate; E12.5 mouse brain lysate. IHC-P: Mouse olfactory epithelium, Nose skin of P0 mouse, Mouse E14.5, Rat olfactory epithelium, Nose skin of P0 rat and rat E14.5 tissues. IHC-Fr: Mouse E14.5 and rat E14.5 tissues. IP: E18 rat brain lysate.
General notes
This product was previously labelled as Lhx2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20449
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded mouse olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse olfactory epithelium [PMID: 15456728].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on vomeronasal organ of mouse E14.5 [PMID: 27521061].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

All lanes : Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution

Lane 1 : His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426)
Lane 2 : His-tagged rat Lhx9 recombinant protein fragment (aa54-172)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 44 kDa
Observed band size: 47 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Lhx2/LH2 was immunoprecipitated from 0.35 mg of E18 rat brain lysate with ab184337 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184337 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: E18 rat brain lysate, 10 μg (Input).

Lane 2: ab184337 IP in E18 rat brain lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184337 in E18 rat brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded Mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of mouse E14.5 [PMID: 25071464].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded rat olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat olfactory epithelium [PMID: 15456728].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on olfactory epithelium of rat E14.5 [PMID: 15456728].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution + E18 rat brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/200 dilution + E12.5 mouse brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Molecular weight observed is consistent with what has been described in the literature, PMID 22028024, PMID 25128627.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded Rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of rat E14.5 [PMID: 25071464].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded nose skin of P0 mouse labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of mouse nose skin [PMID: 20386748].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded nose skin of P0 rat labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of rat nose skin [PMID: 20386748].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

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