Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-4 antibody [EPR22879-236] - BSA and Azide free (ab261917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22879-236] to IL-4 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-IL-4 antibody [EPR22879-236] - BSA and Azide free
    See all IL-4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22879-236] to IL-4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Mouse splenocytes treated with 2.5g/ml concanavalin A for 3 days.

  • General notes

    Ab261917 is the carrier-free version of ab225638. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab261917 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-IL-4 antibody [EPR22879-236] - BSA and Azide free (ab261917)
    Flow Cytometry - Anti-IL-4 antibody [EPR22879-236] - BSA and Azide free (ab261917)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days cells labelling IL-4 with ab225638 at 1/50 dilution.

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody. 

    Cells were surface stained with anti-CD4 conjugated to APC/Fire™ 750, then fixed with 2% PFA for 10min followed by intracellularly stained with anti-IL4 conjugated to PE/Cy7 and rabbit IgG (Left) or ab225248 (Right). 
    Gated on CD4+T cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225638).

  • Anti-IL-4 antibody [EPR22879-236] - BSA and Azide free (ab261917)
    Anti-IL-4 antibody [EPR22879-236] - BSA and Azide free (ab261917)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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