Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1118Y] to IL-4
Suitable for: Flow Cyt, WB, ICC/IF
Reacts with: Human

Product name

Anti-IL-4 antibody [EPR1118Y]
See all IL-4 primary antibodies
Description

Rabbit monoclonal [EPR1118Y] to IL-4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human IL-4 aa 50-150. The exact sequence is proprietary.
Positive control
- Human tonsil lysate
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1118Y
Isotype

IgG
Research areas

Flow Cytometry - Anti-IL-4 antibody [EPR1118Y] (ab62351)

Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)

Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

Control: PBS only
Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)

Anti-IL-4 antibody [EPR1118Y] (ab62351) at 1/2000 dilution + Human tonsil lysate at 10 µg

Secondary
goat anti rabbit HRP at 1/2000 dilution

Predicted band size: 17 kDa
Observed band size: 14 kDa
why is the actual band size different from the predicted?
Anti-IL-4 antibody [EPR1118Y] (ab62351)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com