Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-4 antibody [EPR1118Y] (ab62351)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1118Y] to IL-4
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-4 antibody [EPR1118Y]
    See all IL-4 primary antibodies

  • Description

    Rabbit monoclonal [EPR1118Y] to IL-4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human IL-4 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human tonsil lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-IL-4 antibody [EPR1118Y] (ab62351)
    Flow Cytometry - Anti-IL-4 antibody [EPR1118Y] (ab62351)

    Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)

    Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)
    Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)
    Anti-IL-4 antibody [EPR1118Y] (ab62351) at 1/2000 dilution + Human tonsil lysate at 10 µg

    Secondary
    goat anti rabbit HRP at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?

  • Anti-IL-4 antibody [EPR1118Y] (ab62351)
    Anti-IL-4 antibody [EPR1118Y] (ab62351)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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