Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)
![Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)](https://www.abcam.com/ps/products/246/ab246803/Images/ab246803-3-ab62351275012ab62351FC1.jpg "Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1118Y] to IL-4 - Low endotoxin, Azide free
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free
See all IL-4 primary antibodies -
Description
Rabbit monoclonal [EPR1118Y] to IL-4 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human tonsil lysate
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General notes
ab246803 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: 9% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Endotoxin level is less than 1 EU/ml as determined by the TAL test -
Clonality
Monoclonal -
Clone number
EPR1118Y -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)
Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62351).
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)](https://www.abcam.com/ps/products/246/ab246803/Images/ab246803-2-ab62351262046ab62351Ramosmethanol500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62351).
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![Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)](https://www.abcam.com/ps/products/246/ab246803/Images/ab246803-4-benefits-of-recombinant-antibodies.png)
Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)
