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Cancer Tumor immunology Cytokines Interleukins

Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)

Price and availability

247 929 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5328(2)] to IL-28A + IL-28B
  • Suitable for: WB
  • Reacts with: Recombinant fragment

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Overview

  • Product name

    Anti-IL-28A + IL-28B antibody [EPR5328(2)]
  • Description

    Rabbit monoclonal [EPR5328(2)] to IL-28A + IL-28B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Recombinant fragment
  • Immunogen

    Synthetic peptide within Human IL-28B aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human IL-28A and IL-28B recombinant protein
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5328(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • Immunology
    • Secreted Molecules
    • Other secreted molecules

Images

  • Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)
    Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)
    All lanes : Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/1000 dilution

    Lane 1 : Human IL-28A recombinant protein (aa 27 to 200)
    Lane 2 : House made His-tagged full length human IL-28B recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 22 kDa



    Exposure time

    Lane 1: 40 seconds

    Lane 2: 3 second

    Buffer and concentration: 5% NFDM /TBST

  • Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)
    Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)
    Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/5000 dilution + Human IL-28A (amino acids 27-200) recombinant protein

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?


    Exposure time: 120 seconds


    Blocking buffer and concentration 5% NFDM/TBST
    Diluting buffer and concentration 5% NFDM/TBST

     

  • Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)
    Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)

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    Applications: ICC/IF, WB

  •  
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    Anti-IL-28B antibody (ab189858)

    Applications: WB

  •  
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    Anti-IL-28B antibody (ab229344)

    Applications: WB

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