Recombinant human IL-21 protein (Active) (Biotin) (ab246159)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Alternative names
- CVID11
- IL 21
- IL-21
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Function
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
Tissue specificity
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-21 protein (Active) (Biotin) (ab246159)Immobilized Biotinylated Human IL-21 FcAvitag at 2 µg/mL (100 µL/well) can bind Human IL-21 R (His Tag) with a linear range of 2-8 ng/mL (QC tested).
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SDS-PAGE - Recombinant human IL-21 protein (Active) (Biotin) (ab246159)SDS-PAGE analysis of ab246159 under reducing conditions.
The protein has a calculated MW of 43.9 kDa. The protein migrates as 36 kDa and 48-50 kDa under reducing conditions due to glycosylation.
The gel was stained overnight with Coomassie Blue.
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Functional Studies - Recombinant human IL-21 protein (Active) (Biotin) (ab246159)Immobilized ab246159 at 2 μg/mL (100 μL/well) can bind Human IL-21 R, His Tag with a linear range of 2-8 ng/mL.
