Recombinant human IL-6R protein (ab167742)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 94% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 250 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
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SDS-PAGE - Recombinant human IL-6R protein (ab167742)Human IL-6 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
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Functional Studies - Recombinant human IL-6R protein (ab167742)Immobilized ActiveMax® Human IL-6, Tag Free at 5 µg/mL (100 µL/well) can bind Human IL-6 R alpha, His Tag with a linear range of 1-16 ng/mL. -
SDS-PAGE - Recombinant human IL-6R protein (ab167742)SDS-PAGE analysis of reduced ab167742 and staining overnight with Coomassie Blue. Protein migrates as 60-70 kDa in reduced SDS-PAGE resulting from glycosylation.
