Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 03, 2021

Anti-IL-2 antibody [EPR20055] (ab207325)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20055] to IL-2
Suitable for: WB, ICC/IF, IP
Reacts with: Human, Recombinant fragment

Product name

Anti-IL-2 antibody [EPR20055]
See all IL-2 primary antibodies
Description

Rabbit monoclonal [EPR20055] to IL-2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human IL2 active recombinant protein; Jurkat lysate treated with 40nM Phorbol-12-myristate-13-acetate, 2 µM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight. ICC/IF: Jurkat cells treated with 40 nM Phorbol-12-myristate-13-acetate, 2 µM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight. IP: Jurkat lysate treated with 40nM Phorbol-12-myristate-13-acetate, 2 µM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20055
Isotype

IgG
Research areas

Western blot - Anti-IL-2 antibody [EPR20055] (ab207325)

Anti-IL-2 antibody [EPR20055] (ab207325) at 1/1000 dilution + Human IL2 active recombinant protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 17 kDa
Observed band size: 14 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

Human IL2 active recombinant protein contains aa21-153.
Western blot - Anti-IL-2 antibody [EPR20055] (ab207325)

All lanes : Anti-IL-2 antibody [EPR20055] (ab207325) at 1/5000 dilution

Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with 40nM Phorbol-12-myristate-13-acetate, 2 µM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 17 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with the literature, and the expression level can be induced in activated Jurkat cell. (PMID:10555752).
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR20055] (ab207325)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells, untreated or treated with 40 nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight, labeling IL2 with ab207325 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on Jurkat cells. The signal is induced after treatment with 40 nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only controls on non treated and treated cells: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-IL-2 antibody [EPR20055] (ab207325)

IL2 was immunoprecipitated from 0.35 mg of Jurkat (Human T cell leukemia cell line from peripheral blood) treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight lysate with ab207325 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab207325 at 1/200 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Jurkat treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight lysate 10µg (Input).

Lane 2: ab207325 IP in Jurkat treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight lysate.

Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207325 in Jurkat treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-IL-2 antibody [EPR20055] (ab207325)

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