Anti-TICAM2 antibody [mAbcam77169] (ab77169)
![Anti-TICAM2 antibody [mAbcam77169] (ab77169)](https://www.abcam.com/ps/products/77/ab77169/Images/ab77169-92857-anti-ticam2-antibody-mabcam77169-western-blot.jpg "Anti-TICAM2 antibody [mAbcam77169] (ab77169)")
Key features and details
- Mouse monoclonal [mAbcam77169] to TICAM2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TICAM2 antibody [mAbcam77169]
See all TICAM2 primary antibodies -
Description
Mouse monoclonal [mAbcam77169] to TICAM2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TICAM2 aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab57980) -
Positive control
- Recombinant Human TICAM2 protein (ab123160) can be used as a positive control in WB. This antibody gave a positive signal in Human Kidney and Human Spleen Tissue Lyates
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam77169 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-TICAM2 antibody [mAbcam77169] (ab77169)All lanes : Anti-TICAM2 antibody [mAbcam77169] (ab77169) at 10 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-TICAM2 antibody [mAbcam77169] (ab77169)](https://www.abcam.com/ps/products/77/ab77169/Images/ab77169-92856-anti-ticam2-antibody-mabcam77169-flow-cytometry.jpg)
Flow Cytometry - Anti-TICAM2 antibody [mAbcam77169] (ab77169)Overlay histogram showing PC3 cells stained with ab77169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77169, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
