Human IL-1ra Matched Antibody Pair Kit (ab210888)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Human IL-1ra Matched Antibody Pair Kit
See all IL-1RA kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
5.21 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human IL-1ra Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-1ra.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab211650. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human IL-1ra Capture Antibody 1 x 100µg 1 x 50µg Human IL-1ra Detector Antibody 1 x 25µg 1 x 12.5µg Human IL-1ra Lyophilized Protein 1 vial 1 vial -
Research areas
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Function
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. -
Tissue specificity
The intracellular form of IL1RN is predominantly expressed in epithelial cells. -
Involvement in disease
Microvascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency -
Cellular localization
Cytoplasm and Secreted. - Information by UniProt
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Alternative names
- DIRA
- F630041P17Rik
- ICIL 1RA
see all -
Database links
- Entrez Gene: 3557 Human
- Omim: 147679 Human
- SwissProt: P18510 Human
- Unigene: 81134 Human
Images
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Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human IL-1ra Matched Antibody Pair Kit (ab210888)To learn more about the advantages of recombinant antibodies see here.
