Human IL-20 ELISA Kit (ab231927)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.2 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-20 ELISA Kit
See all IL-20 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Spiked serum 5 3.2% Inter-assay Sample n Mean SD CV% Spiked serum 3 3.5% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.2 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106 102% - 112% Cell culture media 109 105% - 111% Hep Plasma 97 91% - 103% EDTA Plasma 93 89% - 97% Cit plasma 98 96% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-20 ELISA Kit (ab231927) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-20 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-20 with 4.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-20 Capture Antibody 1 x 600µl 10X Human IL-20 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-20 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. -
Tissue specificity
Expressed at very low levels in skin, trachea, and other tissues. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine Zcyto10
- Four alpha helix cytokine
- IL-20
see all -
Database links
- Entrez Gene: 50604 Human
- Omim: 605619 Human
- SwissProt: Q9NYY1 Human
- Unigene: 272373 Human
Images
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Other - Human IL-20 ELISA Kit (ab231927)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-20 Competitor standard curve comparisonStandard curve comparison between Human IL-20 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human IL-20 standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked IL-20 in human serum and plasma samplesThe concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Serum from ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). No detectable levels of IL-20 were measured.
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Interpolated concentrations of spiked IL-20 in human cell culture media sampleThe concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human IL-20 ELISA Kit (ab231927)To learn more about the advantages of recombinant antibodies see here.
