Anti-pro Caspase-10 antibody [E35] (ab32155)
![Anti-pro Caspase-10 antibody [E35] (ab32155)](https://www.abcam.com/ps/products/32/ab32155/Images/ab32155-44060-anti-pro-caspase-10-antibody-e35-western-blot.gif "Anti-pro Caspase-10 antibody [E35] (ab32155)")
Key features and details
- Rabbit monoclonal [E35] to pro Caspase-10
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-pro Caspase-10 antibody [E35] -
Description
Rabbit monoclonal [E35] to pro Caspase-10 -
Host species
Rabbit -
Specificity
The antibody does not crossreact with other Caspase family members. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human pro Caspase-10 aa 200-300 (N terminal). The exact sequence is proprietary.
-
Positive control
- WB: Hela cell lysate. IF and FACS: Hela cells. IHC-P: Human breast carcinoma tissue.
-
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
E35 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-pro Caspase-10 antibody [E35] (ab32155)Anti-pro Caspase-10 antibody [E35] (ab32155) at 1/1000 dilution + Hela cell lysate
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-10 antibody [E35] (ab32155)](https://www.abcam.com/ps/products/32/ab32155/Images/ab32155-44059-anti-pro-caspase-10-antibody-e35-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-10 antibody [E35] (ab32155)Ab32155, at 1/50 dilution, staining pro Caspase 10 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-pro Caspase-10 antibody [E35] (ab32155)](https://www.abcam.com/ps/products/32/ab32155/Images/ab32155-44058-anti-pro-caspase-10-antibody-e35-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pro Caspase-10 antibody [E35] (ab32155)Ab32155, at 1/100 dilution, staining pro Caspase 10 in apoptotic HeLa cells by Immunofluorescence. -
![Flow Cytometry - Anti-pro Caspase-10 antibody [E35] (ab32155)](https://www.abcam.com/ps/products/32/ab32155/Images/ab32155-44057-anti-pro-caspase-10-antibody-e35-flow-cytometry.gif)
Flow Cytometry - Anti-pro Caspase-10 antibody [E35] (ab32155)Flow cytometric analysis of permeabilized HeLa cells using ab32155 at a 1/50 dilution (red) or a rabbit IgG (negative)(green).
