Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16805-15] to IL-1 beta - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free
    See all IL-1 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR16805-15] to IL-1 beta - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).

     

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: RAW 264.7 treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h, whole cell lysate. Flow cyt: RAW 264.7 cells treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h.

  • General notes

    Ab254195 is the carrier-free version of ab234437. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254195 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)
    Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)

    IL-1 beta was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate with ab234437 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234437 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate 10 μg (Input).
    Lane 2: ab234437 IP in RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234437 in RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate.

    IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).

     

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234437).

  • Flow Cytometry - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)
    Flow Cytometry - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells that were either treated (100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h labeling)(red) or untreated (green) labeling IL-1 beta with ab234437 at 1/60 dilution compared with a rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti-Rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234437).

  • Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)
    Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)

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