Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23693-37] to IL-12RB1 - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free
    See all IL-12RB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23693-37] to IL-12RB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon, tonsil and diffuse large B-lymphoma tissue; Mouse colon tissue; Rat colon tissue. IHC-Fr: mouse lymph node and colon tissue.

  • General notes

    ab280892 is the carrier-free version of ab256805. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280892 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23693-37

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

    This data was developed using ab256805, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IL-12RB1 with ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes of human colon. The section was incubated with ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
    Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

    This data was developed using ab256805, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Perfused-fixed mouse lymph node tissue labeling IL-12RB1 with ab256805 at 1/50 (11.38 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lymph node. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

    This data was developed using ab256805, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IL-12RB1 with ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil (PMID: 11120812). The section was incubated with ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
    Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

    This data was developed using ab256805, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Perfused-fixed mouse colon tissue labeling IL-12RB1 with ab256805 at 1/50 (11.38 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on lymphocytes of mouse colon. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

    This data was developed using ab256805, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma tissue labelling IL-12RB1 with ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human diffuse large B-cell lymphoma. The section was incubated with ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

    This data was developed using ab256805, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling IL-12RB1 with ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes of mouse colon. The section was incubated with ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

    This data was developed using ab256805, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling IL-12RB1 with ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on lymphocytes of rat colon. The section was incubated with ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
    Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)

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