Anti-IKZF3 Degron tag antibody [1C3] (ab269464)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [1C3] to IKZF3 Degron tag
- Suitable for: WB, ELISA
- Reacts with: Human
Overview
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Product name
Anti-IKZF3 Degron tag antibody [1C3]
See all IKZF3 Degron tag primary antibodies -
Description
Mouse monoclonal [1C3] to IKZF3 Degron tag -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate. ELISA: Human IKZF3 Degron tag.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1C3 -
Isotype
IgG1
Images
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All lanes : Anti-IKZF3 Degron tag antibody [1C3] (ab269464) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-mCherry, whole cell lysate
Lane 2 : HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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ab269464 used at 0-4000 ng/ml.
Antigen (Human IKZF3 Degron tag) used at 1000 ng/ml.
Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.
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