Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP105] to IGJ - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-IGJ antibody [SP105] - BSA and Azide free
    See all IGJ primary antibodies

  • Description

    Rabbit monoclonal [SP105] to IGJ - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and stomach tissue

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab240939 is the carrier-free version of ab105229. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240939 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP105

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab105229 for 10 mins at room temperature.

    This image was generated using ab105229, the same clone, but with a different buffer formulation.

  • Flow Cytometry - Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)
    Flow Cytometry - Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)

    Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) labeling IGJ with purified ab105229 at 1/20 dilution (5.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab105229).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells of human stomach, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab105229 for 10 mins at room temperature.

    This image was generated using ab105229, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)

    ab105229, at 1/100 dilution, staining IGJ in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab105229)

  • Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)
    Anti-IGJ antibody [SP105] - BSA and Azide free (ab240939)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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