Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-IGJ antibody [EPR23130-113] (ab269855)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23130-113] to IGJ
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-IGJ antibody [EPR23130-113]
See all IGJ primary antibodies
Description

Rabbit monoclonal [EPR23130-113] to IGJ
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human tonsil tissue lysate; Daudi, Raji and Ramos cell lysate. IHC-P: Human bladder cancer, spleen and colon tissue. ICC/IF: Daudi cells. IP: Human tonsil tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23130-113
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-IGJ antibody [EPR23130-113] (ab269855)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi cells labeling IGJ with ab224687 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Daudi cells is observed. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] (ab269855)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human bladder cancer is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] (ab269855)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human colon (PMID:12389099) is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] (ab269855)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human spleen (PMID:12389099) is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunoprecipitation - Anti-IGJ antibody [EPR23130-113] (ab269855)

IGJ was immunoprecipitated from 0.35 mg human tonsil lysate with ab269855 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 269855 1/1000 dilution (0.48 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Human tonsil lysate 10 μg
Lane 2: ab269855 IP in human tonsil lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269855 in human tonsil lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
Western blot - Anti-IGJ antibody [EPR23130-113] (ab269855)

All lanes : Anti-IGJ antibody [EPR23130-113] (ab269855) at 1/1000 dilution

Lane 1 : Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
Lane 3 : Ramos (human burkitt's lymphoma b lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 18 kDa

Blocking/diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lane 1: 26 secs; Lanes 2-3: 6 secs.
Western blot - Anti-IGJ antibody [EPR23130-113] (ab269855)

Anti-IGJ antibody [EPR23130-113] (ab269855) at 1/1000 dilution + Human tonsil lysate at 10 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 18 kDa

The expression molecular weight observed is consistent with what has been described in the literature (PMID:8078886).

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure Time: 81 seconds.
Anti-IGJ antibody [EPR23130-113] (ab269855)

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