Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HSPA14 antibody [EPR4210] (ab108612)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4210] to HSPA14
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human, African green monkey

Overview

  • Product name

    Anti-HSPA14 antibody [EPR4210]
    See all HSPA14 primary antibodies

  • Description

    Rabbit monoclonal [EPR4210] to HSPA14

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, COS1, Human fetal liver, and HeLa cell lysates. Human kidney tissue. HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4210

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HSPA14 antibody [EPR4210] (ab108612)
    Western blot - Anti-HSPA14 antibody [EPR4210] (ab108612)
    All lanes : Anti-HSPA14 antibody [EPR4210] (ab108612) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : COS-1 (African green monkey kidney fibroblast-like cell line) cell lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 55 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] (ab108612)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] (ab108612)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] (ab108612)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] (ab108612)
    Immunofluorescent staining of HeLa cells using ab108612 at 1/100
  • Anti-HSPA14 antibody [EPR4210] (ab108612)
    Anti-HSPA14 antibody [EPR4210] (ab108612)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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