Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab194677)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K36) - ChIP Grade
- Suitable for: Dot blot, WB, ICC/IF, ChIP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K36) - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanICC/IF HumanIHC-P MouseRatHumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K36).
Database link: Q16695 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K36) antibody (ab194677)
Paraffin-embedded human breast tissue stained for Histone H3 (tri methyl K36) using ab194677 at 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody (ab194677)Immunofluorescence staining of 293T cells stained for Histone H3 (tri methyl K36) with ab194677. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-Histone H3 (tri methyl K36) antibody (ab194677)All lanes : Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab194677) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : C2C12 cell lysate
Lane 3 : H3 protein
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 16 kDa
Exposure time: 1 secondBlocking buffer: 3% nonfat dry milk in TBST.
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Dot Blot - Anti-Histone H3 (tri methyl K36) antibody (ab194677)Dot-blot analysis of all sorts of methylation peptides using ab194677.
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ChIP - Anti-Histone H3 (tri methyl K36) antibody (ab194677)Chromatin immunoprecipitation analysis of extracts of 293T cells, using ab194677 and rabbit IgG. P1 and P2 were located on GAPDH gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K36) antibody (ab194677)Paraffin-embedded mouse testis tissue stained for Histone H3 (tri methyl K36) using ab194677 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K36) antibody (ab194677)Paraffin-embedded rat testis tissue stained for Histone H3 (tri methyl K36) using ab194677 at 1/200 dilution in immunohistochemical analysis.
