Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
![Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)](https://www.abcam.com/ps/products/184/ab184171/Images/ab184171-450477-anti-aldehyde-dehydrogenase-10-antibody-epr15425b-western-blot-wildetype-HeLa-aldh3a2-knockout-HeLa.jpg "Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10
- Suitable for: WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)]
See all Aldehyde dehydrogenase 10 primary antibodies -
Description
Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A549, 293T and U87-MG cell lysates; A549 and HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15425(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ALDH3A2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab184171 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab184171 was shown to react with ALDH3A2 in wild-type HeLa cells in Western blot with loss of signal observed in ALDH3A2 knockout cell line ab265427 (ALDH3A2 knockout cell lysate ab257829). Wild-type and ALDH3A2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab184171 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)](https://www.abcam.com/ps/products/184/ab184171/Images/ab184171-216410-anti-aldehyde-dehydrogenase-10-antibody-epr15425b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/250 dilution (red). A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).
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![Flow Cytometry - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)](https://www.abcam.com/ps/products/184/ab184171/Images/ab184171-216411-anti-aldehyde-dehydrogenase-10-antibody-epr15425b-flow-cytometry.jpg)
Flow Cytometry - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)Flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/170 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Isotype control: Rabbit monoclonal IgG (green).
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![Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)](https://www.abcam.com/ps/products/184/ab184171/Images/ab184171-216413-anti-aldehyde-dehydrogenase-10-antibody-epr15425b-western-blot.jpg)
Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/10000 dilution + U87-MG cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
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![Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)](https://www.abcam.com/ps/products/184/ab184171/Images/ab184171-216412-anti-aldehyde-dehydrogenase-10-antibody-epr15425b-western-blot.jpg)
Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/2000 dilution
Lane 1 : A549 cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
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![Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)](https://www.abcam.com/ps/products/184/ab184171/Images/ab184171-5-benefits-of-recombinant-antibodies.png)
Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)