Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)]
    See all Aldehyde dehydrogenase 10 primary antibodies

  • Description

    Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, A549, 293T and U87-MG cell lysates; A549 and HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ALDH3A2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab184171 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab184171 was shown to react with ALDH3A2 in wild-type HeLa cells in Western blot with loss of signal observed in ALDH3A2 knockout cell line ab265427 (ALDH3A2 knockout cell lysate ab257829). Wild-type and ALDH3A2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab184171 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

    Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/250 dilution (red). A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).

  • Flow Cytometry - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    Flow Cytometry - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

    Flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/170 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Isotype control: Rabbit monoclonal IgG (green).

  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/10000 dilution + U87-MG cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/2000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)
    Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

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