Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6725] to NDUFAB1
  • Suitable for: IP, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFAB1 antibody [EPR6725]
    See all NDUFAB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6725] to NDUFAB1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Molt-4, A431 and HepG2 cell lysate. Human heart and kidney tissue. HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
    Western blot - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
    All lanes : Anti-NDUFAB1 antibody [EPR6725] (ab181021) at 1/10000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

    Immunohistochemical analysis of paraffin embedded Human heart tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
    Flow Cytometry - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

    Flow Cytometry analysis of HepG2 cells labeling NDUFAB1 using ab181021 at a 1/160 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Immunoprecipitation - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
    Immunoprecipitation - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

    Lysate from HepG2 cells was immunoprecipitated with ab181021 at a 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

  • Anti-NDUFAB1 antibody [EPR6725] (ab181021)
    Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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