Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6725] to NDUFAB1
Suitable for: IP, Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-NDUFAB1 antibody [EPR6725]
See all NDUFAB1 primary antibodies
Description

Rabbit monoclonal [EPR6725] to NDUFAB1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Molt-4, A431 and HepG2 cell lysate. Human heart and kidney tissue. HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6725
Isotype

IgG
Research areas

Western blot - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

All lanes : Anti-NDUFAB1 antibody [EPR6725] (ab181021) at 1/10000 dilution

Lane 1 : Molt-4 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Immunohistochemical analysis of paraffin embedded Human heart tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Flow Cytometry analysis of HepG2 cells labeling NDUFAB1 using ab181021 at a 1/160 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Immunoprecipitation - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Lysate from HepG2 cells was immunoprecipitated with ab181021 at a 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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