Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23235-147] to Acetyl Coenzyme A carboxylase alpha - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free
    See all Acetyl Coenzyme A carboxylase alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR23235-147] to Acetyl Coenzyme A carboxylase alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T, HeLa, PC-12 and C2C12 lysates. IHC-P: Human breast carcinoma and endometrial carcinoma tissue. Mouse and rat lung tissue. ICC/IF: C2C12 and 293T cells. Flow Cyt: C2C12 and 293T cells. IP: C2C12 and 293T cells.

  • General notes

    ab272704 is the carrier-free version of ab269273. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Acetyl Coenzyme A carboxylase alpha was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast), whole cell lysate 10 µg with ab269273 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269273 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: C2C12 whole cell lysate 10 µg.

    Lane 2: ab269273 IP in C2C12 whole cell lysate 10 µg.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269273 in C2C12 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Acetyl Coenzyme A carboxylase alpha was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell), whole cell lysate 10 µg with ab269273 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269273 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: 293T whole cell lysate 10 µg.

    Lane 2: ab269273 IP in 293T whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269273 in 293T whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Flow Cytometry - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Flow Cytometry - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling Acetyl Coenzyme A carboxylase alpha with ab269273 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Flow Cytometry - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Flow Cytometry - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling Acetyl Coenzyme A carboxylase alpha with ab269273 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T cells labelling Acetyl Coenzyme A carboxylase alpha with ab269273 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in 293T cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Acetyl Coenzyme A carboxylase alpha with ab269273 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 2 µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269273 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat lung (PMID: 17521700). The section was incubated with ab269273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269273 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung (PMID: 17521700). The section was incubated with ab269273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269273 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human endometrial carcinoma. The section was incubated with ab269273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269273 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast carcinoma (PMID: 21415164). The section was incubated with ab269273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269273).

  • Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)
    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-147] - BSA and Azide free (ab272704)

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