Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)
![Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)](https://www.abcam.com/ps/products/251/ab251224/Images/ab251224-408100-anti-histone-h3-methyl-r63-antibody-epr17722-bsa-and-azide-free-western-blot.jpg "Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17722] to Histone H3 (methyl R63) - BSA and Azide free
- Suitable for: PepArr, WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free
See all Histone H3 primary antibodies -
Description
Rabbit monoclonal [EPR17722] to Histone H3 (methyl R63) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: PepArr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251224 is the carrier-free version of ab196724 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251224 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
R
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17722 -
Isotype
IgG
Images
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Western blot - Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 1 minuteThis data was developed using ab196724, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA /TBST.
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![Peptide Array - Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)](https://www.abcam.com/ps/products/251/ab251224/Images/ab251224-3-anti-histone-h3-methyl-r63-antibody-epr17722-bsa-and-azide-free-peptide-array.jpg)
Peptide Array - Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)This data was developed using ab196724, the same antibody clone in a different buffer formulation.ab196724 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Western blot - Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)](https://www.abcam.com/ps/products/251/ab251224/Images/ab251224-403463-anti-histone-h3-methyl-r63-antibody-epr17722-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724) at 1/1000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3 minutesThis data was developed using ab196724, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)](https://www.abcam.com/ps/products/251/ab251224/Images/ab251224-1-anti-histone-h3-methyl-r63-antibody-epr17722-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)This data was developed using ab196724, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Histone H3 (methyl R63, asymmetric di methyl R63, symmetric di methyl R63) with ab196724 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. -
![Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)](https://www.abcam.com/ps/products/251/ab251224/Images/ab251224-5-benefits-of-recombinant-antibodies.png)
Anti-Histone H3 (methyl R63) antibody [EPR17722] - BSA and Azide free (ab251224)
