Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
![Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)](https://www.abcam.com/ps/products/209/ab209965/Images/ab209965-405497-anti-rsk1-p90-phospho-t573-antibody-ep2133y-bsa-and-azide-free-western-blot.jpg "Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573) - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free
See all RSK1 p90 primary antibodies -
Description
Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562 cell lysate.
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General notes
ab209965 is the carrier-free version of ab62324 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab209965 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2133Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/2000 dilution (purified)
Lane 1 : Untreated Ramos whole cell lysate
Lane 2 : Ramos treated with Phorbol-12-myristate-13-acetate whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaThis data was developed using ab62324, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)](https://www.abcam.com/ps/products/209/ab209965/Images/ab209965-2-anti-rsk1-p90-phospho-t573-antibody-ep2133y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)This data was developed using ab62324, the same antibody clone in a different buffer formulation.Immunofluorescence staining of K562 cells with purified ab62324 at a working dilution of 1/500, counter-stained with DAPI. The cells were treated with 500 nM TPA for 15 minutes. Both the treated and untreated cells are shown. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the right hand panels. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom panels - for negative control 1, purified ab62324 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400. -
![Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)](https://www.abcam.com/ps/products/209/ab209965/Images/ab209965-397900-anti-rsk1-p90-phospho-t573-antibody-ep2133y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/500 dilution (Unpurified)
Lane 1 : K562 cell lysate; untreated
Lane 2 : K562 cell lysate; treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaThis data was developed using ab62324, the same antibody clone in a different buffer formulation.
Beta Tubulin has been included as a loading control.
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![Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)](https://www.abcam.com/ps/products/209/ab209965/Images/ab209965-4-benefits-of-recombinant-antibodies.png)
Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)