Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573) - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free
    See all RSK1 p90 primary antibodies

  • Description

    Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562 cell lysate.

  • General notes

    ab209965 is the carrier-free version of ab62324 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab209965 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
    Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
    All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/2000 dilution (purified)

    Lane 1 : Untreated Ramos whole cell lysate
    Lane 2 : Ramos treated with Phorbol-12-myristate-13-acetate whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    This data was developed using ab62324, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
    Immunocytochemistry - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
    This data was developed using ab62324, the same antibody clone in a different buffer formulation.Immunofluorescence staining of K562 cells with purified ab62324 at a working dilution of 1/500, counter-stained with DAPI. The cells were treated with 500 nM TPA for 15 minutes. Both the treated and untreated cells are shown. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the right hand panels. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom panels - for negative control 1, purified ab62324 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
    Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
    All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/500 dilution (Unpurified)

    Lane 1 : K562 cell lysate; untreated
    Lane 2 : K562 cell lysate; treated with TPA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    This data was developed using ab62324, the same antibody clone in a different buffer formulation.

    Beta Tubulin has been included as a loading control.

  • Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
    Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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