Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T cell lysate.

  • General notes

    Ab247882 is the carrier-free version of ab109501. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247882 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)
    All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PLCG1 knockout HEK-293T cell lysate

    Lysates/proteins at 40 µg/ml per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

    Predicted band size: 149 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab109501).

    Lanes 1- 2: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

    ab109501 was shown to react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type HEK-293T and PLCG1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)
    Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)

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