Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)
![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)](https://www.abcam.com/ps/products/247/ab247882/Images/ab247882-395791-anti-phospholipase-c-gamma-1plc-gamma-1-antibody-epr5358-western-blot-hek-293t-lysates.jpg "Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details
Unsuitable for: ICC,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T cell lysate.
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General notes
Ab247882 is the carrier-free version of ab109501. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247882 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5358 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PLCG1 knockout HEK-293T cell lysate
Lysates/proteins at 40 µg/ml per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 149 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab109501).
Lanes 1- 2: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab109501 was shown to react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type HEK-293T and PLCG1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)](https://www.abcam.com/ps/products/247/ab247882/Images/ab247882-2-benefits-of-recombinant-antibodies.png)
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)
