Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1832Y] to FAK (phospho Y576 + Y577) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free
    See all FAK primary antibodies

  • Description

    Rabbit monoclonal [EP1832Y] to FAK (phospho Y576 + Y577) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Detects FAK phosphorylated at Y576/577.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247455 is the carrier-free version of ab76244 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247455 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)
    Dot Blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)
    This data was developed using ab76244, the same antibody clone in a different buffer formulation.Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) Dot Blot. Primary ab dilution 1:1000, Secondary ab description and code (ab id), Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000, Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST, Lane 1:FAK (pY576+pY577) phospho peptide, Lane 2:FAK (pY576) phospho peptide, Lane 3:FAK (pY577) phospho peptide, Lane 4:FAK non-phospho peptide, Exposure time 3 minutes. Note: antibody used for ab76244 is purified batch.
  • Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)
    Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)
    All lanes : Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) at 1/50000 dilution

    Lane 1 : NIH-3T3 cell lysates, untreated
    Lane 2 : NIH-3T3 cell lysates treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size: 119 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76244, the same antibody clone in a different buffer formulation.

     

  • Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)
    Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)

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