Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)
![Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)](https://www.abcam.com/ps/products/247/ab247455/Images/ab247455-2-anti-fak-phospho-y576-y577-antibody-ep1832y-bsa-and-azide-free-dot-blot.jpg "Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1832Y] to FAK (phospho Y576 + Y577) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free
See all FAK primary antibodies -
Description
Rabbit monoclonal [EP1832Y] to FAK (phospho Y576 + Y577) - BSA and Azide free -
Host species
Rabbit -
Specificity
Detects FAK phosphorylated at Y576/577.
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Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247455 is the carrier-free version of ab76244 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247455 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1832Y -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)This data was developed using ab76244, the same antibody clone in a different buffer formulation.Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) Dot Blot. Primary ab dilution 1:1000, Secondary ab description and code (ab id), Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000, Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST, Lane 1:FAK (pY576+pY577) phospho peptide, Lane 2:FAK (pY576) phospho peptide, Lane 3:FAK (pY577) phospho peptide, Lane 4:FAK non-phospho peptide, Exposure time 3 minutes. Note: antibody used for ab76244 is purified batch.
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![Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)](https://www.abcam.com/ps/products/247/ab247455/Images/ab247455-397952-anti-fak-phospho-y576-y577-antibody-ep1832y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)All lanes : Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) at 1/50000 dilution
Lane 1 : NIH-3T3 cell lysates, untreated
Lane 2 : NIH-3T3 cell lysates treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?This data was developed using ab76244, the same antibody clone in a different buffer formulation.
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![Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)](https://www.abcam.com/ps/products/247/ab247455/Images/ab247455-3-benefits-of-recombinant-antibodies.png)
Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] - BSA and Azide free (ab247455)
