Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAD51AP1 antibody (ab101321)

Key features and details

  • Rabbit polyclonal to RAD51AP1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab101321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Cow
Dog
Pig
Macaque monkey
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

  • Function

    May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.

  • Tissue specificity

    Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus. Colocalizes with RAD51 to multiple nuclear foci.
  • Information by UniProt

  • Database links

    • Alternative names

      • 2510006L10Rik antibody
      • MGC156698 antibody
      • OTTMUSP00000028651 antibody
      • PIR51 antibody
      • R51A1_HUMAN antibody
      • RAB22 antibody
      • RAD51 associated protein 1 antibody
      • RAD51 interacting protein antibody
      • RAD51-associated protein 1 antibody
      • RAD51-interacting protein antibody
      • Rad51ap1 antibody
      see all

    Images

    • Western blot - Anti-RAD51AP1 antibody (ab101321)
      Western blot - Anti-RAD51AP1 antibody (ab101321)
      All lanes : Anti-RAD51AP1 antibody (ab101321) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
      Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 38 kDa
      Observed band size: 38 kDa
      Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes

    References (2)

    Publishing research using ab101321? Please let us know so that we can cite the reference in this datasheet.

    ab101321 has been referenced in 2 publications.

    • Wu Y  et al. Silencing of RAD51AP1 suppresses epithelial-mesenchymal transition and metastasis in non-small cell lung cancer. Thorac Cancer 10:1748-1763 (2019). PubMed: 31317661
    • Cukras S  et al. The USP1-UAF1 complex interacts with RAD51AP1 to promote homologous recombination repair. Cell Cycle 15:2636-2646 (2016). PubMed: 27463890

    Images

    • Western blot - Anti-RAD51AP1 antibody (ab101321)
      Western blot - Anti-RAD51AP1 antibody (ab101321)
      All lanes : Anti-RAD51AP1 antibody (ab101321) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
      Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 38 kDa
      Observed band size: 38 kDa
      Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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